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CASE REPORT
Year : 2008  |  Volume : 11  |  Issue : 4  |  Page : 245-247
 

Meningitis caused by Rhodotorula mucilaginosa in human immunodeficiency virus seropositive patient


Department of Microbiology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai, India

Date of Submission03-May-2008
Date of Decision01-May-2008
Date of Acceptance16-May-2008

Correspondence Address:
V P Baradkar
Department of Microbiology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai-400022
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-2327.44561

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   Abstract 

Rhodotorula species may be responsible for systemic infection in immunocompromised patients. Meningitis by Rhodotorula species in human immunodeficiency virus (HIV) infected persons has been reported previously. We report a case of meningitis caused by Rhodotorula mucilaginosa in a 36-year-old HIV seropositive male patient who presented with fever, altered sensorium and features of meningeal irritation i.e. neck rigidity. The Cerebrospinal fluid (CSF) cell counts were high, showing 150 cells/mm 3 , with 60% lymphocytes and 40% polymorphs, and protein content of 100 mg%; glucose was 60 mg%. The diagnosis was confirmed by culture on Sabouraud's Dextrose agar. The patient was treated successfully with intensive Amphotericin B (1 mg/kg), for two weeks, followed by oral Itraconazole (400 mg daily), for a period of two months. The patient was started on anti retroviral therapy. He did not show any relapse of the symptoms when the last follow up was done six months after the date of discharge.


Keywords: Human immunodeficiency virus, meningitis, Rhodotorula


How to cite this article:
Baradkar V P, Kumar S. Meningitis caused by Rhodotorula mucilaginosa in human immunodeficiency virus seropositive patient. Ann Indian Acad Neurol 2008;11:245-7

How to cite this URL:
Baradkar V P, Kumar S. Meningitis caused by Rhodotorula mucilaginosa in human immunodeficiency virus seropositive patient. Ann Indian Acad Neurol [serial online] 2008 [cited 2019 Jul 24];11:245-7. Available from: http://www.annalsofian.org/text.asp?2008/11/4/245/44561



   Introduction Top


Invasive fungal disease continues to be a significant problem among immunocompromised patients. Most fungal infections are caused by commonly recognized opportunistic fungi such as Aspergillus species, Candida species, Cryptococcus neoformans and dimorphic fungi. [1] Fungi like Trichosporon beigelii , Malassezia furfur, Geotrichum candidum and Rhodotorula species are emerging pathogens of immunocompromised patients. [1],[2],[3] Rhodotorula species has been reported to cause septicemia, sepsis, meningitis, endocarditis, keratitis, ventriculitis, peritonitis, central venous catheter infection and endophthalmitis. [3],[4],[5],[6],[7],[8],[9],[10] We report a case of meningitis caused by Rhodotorula mucilaginosa in an HIV infected patient.


   Case History Top


A 36-year-old male patient presented with a history of headache and fever for the past seven days, and altered sensorium since the last three days. On enquiry, it was found that he had had pulmonary tuberculosis two years back, for which he had received anti tubercular therapy comprising of 2H 3 R 3 Z 3 E 3 (H: Isoniazid (600 mg), R: Rifampicin (450 mg), Z: Pyrazinamide (1500 mg), Ethambutol (1200 mg) for two months, followed by 4H 3 R 3 (H: Isoniazid (600 mg), R: Rifampicin (450 mg), for a period of four months.

On general examination the patient was found to be febrile (100o F); there was no pallor, icterus or lymphadenopathy noted.

On neurological examination, the patient was found to have altered sensorium and neck rigidity. Other motor and sensory examinations were unremarkable. The cardiovascular and respiratory system examinations were within normal limits. Abdominal examination per se did not reveal any guarding, rigidity, free fluid or organomegaly.

The patient's laboratory investigations revealed hemoglobin (Hb) of 12 gm%, erythrocyte sedimentation rate (ESR) of 8 mm/hr, total leukocyte count (TLC) - 7600/mm 3 , with lymphocytes 60% and polymorphs 40%. The liver and kidney functions were within normal limits. An X-ray of the chest did not reveal any abnormality. Serological testing for HIV antibodies was performed using Enzyme linked immunosorbent assay (ELISA), which revealed that he was reactive to HIV 1 antibodies. The CD 4 counts were 196 cells/mm 3 ; however, viral load and computerized tomography (CT) scan of the brain was not performed.

In view of the altered sensorium and neck rigidity, a lumbar puncture was performed. The cerebrospinal fluid (CSF) was examined by microscopy, with 10% Nigrosin staining, Gram staining, and Ziehl-Neelsen staining, and bacteriological and fungal culture was done. The CSF cell counts were high, showing 150 cells/mm 3 , with 60% lymphocytes & 40% polymorphs, protein content of 100 mg%; glucose was 60 mg%. Nigrosin staining showed encapsulated budding yeast cells [Figure 1]. Gram stained smear showed Gram positive budding yeast cells. Initially, presumptive diagnosis of cryptococcal meningitis was made on the basis of Nigrosin mount and Gram staining. Ziehl-Neelsen staining was negative for acid fast bacilli. No organisms grew on bacteriological culture of CSF. A red pasty mucoid growth [Figure 2] was seen on CSF culture on Sabouraud's Dextrose agar with antibiotics (Chloramphenicol 0.05 mg/L, cycloheximide 0.5 mg/L) and without antibiotics at 25°C and 37°C. Gram staining from colony revealed Gram positive budding yeast cells. These findings, along with the biochemical tests [11] and the absence of ballistospore formation, helped us to diagnose the pathogen as Rhodotorula mucilaginosa and the meningitis as a case of Rhodotorula meningitis. The possibility of contamination was ruled out by repeated isolation from another CSF sample of the patient, collected aseptically, two days after the initial report of capsulated yeasts seen in the CSF was given. The blood cultures of the patient, however, showed no growth in culture.

The patient was administered intravenous Amphotericin B (1 mg/kg/day) for two weeks. He responded well to the treatment, with the fever subsiding after one week of Amphotericin B therapy; he gradually regained consciousness. The patient was later started on oral Itraconazole (400 mg daily) for a period of two months. The patient was discharged after a period of four weeks from the date of admission and was started on anti retroviral therapy namely Zidovudine (300 mg) + Lamivudine (150 mg) + Nevirapine (200 mg), because of his low CD 4 counts. The patient had not shown any relapse of symptoms when the last follow up was done six months after the date of discharge.


   Discussion Top


The incidence of fungal infections of Central nervous system (CNS) has shown a steep rise, largely due to the advent of acquired immune deficiency syndrome (AIDS), and the widespread use of broad spectrum antibiotics, steroids and immunosuppressive drugs. [4] Rhodotorula species is emerging in immune compromised patients, especially in HIV infected patients. [1],[2],[3] Rhodotorula species is common saprophytes in the environment. However, their etiological role in immunocompromised patients has been described only rarely. [1],[2],[3]

Rhodotorula fungal infections of the CNS continue to be uncommon. Rhodotorula belongs to the family Sporidiobolaceae and Phylum Basidiomycota. [11] It produces mucoid yeast like soft, pasty colonies with red pigment on Sabouraud's Dextrose agar (SDA) medium. Microscopically, the unicellular cells of this fungus are spherical in shape, size varying from 4 mm to 6 mm, surrounded by a capsule. No ascospores are present. Many species of Rhodotorula have been described, but Rhodotorula mucilaginosa is the commonest species isolated from clinical specimens. [1],[2],[3] Rhodotorula species and Cryptococcus neoformans have many similarities and both produce meningitis. Hence each may be mistaken for the other. Rhodotorula species differs from Cryptococcus neoformans in its carotenoid pigments. [1],[2],[3],[4] Its isolation from normally sterile sites such as blood and CSF is of greater significance. [5] Rhodotorula species has been reported to cause septicemia, sepsis, meningitis, endocarditis, keratitis, ventriculitis, peritonitis, central venous catheter infection and endophthalmitis. [3],[4],[6],[10],[12],[13] Pore and Chen [14] reported a case of Rhodotorula mucilaginosa meningitis in patients with acute lymphoblastic leukemia. He detected encapsulated budding yeast cells and confirmed it by culture.

A case of meningitis in HIV infected persons was reported by Gyaurgieva et al . [6] Recently Thakur et al. [4] described Rhodotorula mucaliginosa meningitis in an HIV infected patient from Himachal Pradesh, India. The patient was initially on intravenous Amphotericin B; later, intravenous Flucytosine (25 mg/kg) was added. However, in spite of the treatment, the patient died. On the other hand, our patient responded to intravenous Amphotericin B treatment. A study of the susceptibility profile of 29 clinical isolates of Rhodotorula spp. revealed that Fluconazole, itraconazole and voriconazole were inactive in vitro against a majority of Rhodotorula isolates, while Amphotericin B and flucytosine exhibit good activity, being reasonable alternatives for empirical treatment. [15] The new and investigational triazoles all have some in vitro activity, with ravuconazole being the most active. [10] Treatment of Rhodotorula infection involved the removal of central venous catheters if any, and, generally, amphotericin B or fluconazole therapy for 14 days. [11]

The recognition of unusual yeasts as agents of occasional life-threatening infections and their unpredictable antifungal susceptibilities increases the burden on the clinical microbiology laboratory to complete species identification and determine minimal inhibitory concentration (MIC).

To conclude, Rhodotorula species should be kept in mind while diagnosing and treating cases of meningitis, especially in HIV infected patients.

 
   References Top

1.Lanzafame M, De Checchi G, Parinello A, TrevenZoli M, Catlelan AM. Rhodotorula glutinis related meningitis. J Clin Microbiol 2001;39:410.  Back to cited text no. 1    
2.El-Tahawy AT, Khalaf RM. Rhodotorula fungemia in an immuno compromised patient. Ann Saudi Med 1999;19:533-5.  Back to cited text no. 2  [PUBMED]  
3.Naveh Y, Friedman A, Merzbaeh D, Hashman N. Endocarditis caused by Rhodotorula successfully treated with 5- flucytosine. Br Heart J 1975;37:101-4.  Back to cited text no. 3    
4.Thakur K, Singh G, Agarwal S, Rani L. Meningitis caused by Rhodotorula in an Human Immunodeficiency virus infected patient. Indian J Med Microbiol 2007;25:166-8.  Back to cited text no. 4  [PUBMED]  Medknow Journal
5.Rippon JW, Medical Microbiology. The pathogenic fungi and pathogenic Actinomycetes. 3rd ed. New York: W. B. Saunders Company; 1988. p. 582-609.  Back to cited text no. 5    
6.Gyaurgieva OH, Bogomolova TS, Gorshkova GI. Meningitis caused by Rhodotorula rubra in an HIV- infected patient. J Med Vet Mycol 1996;34:357-9.  Back to cited text no. 6  [PUBMED]  
7.Gregory JK, Haller JA. Chronic postoperative Rhodotorula endophthalmitis. Arch Ophthalmol 1992;110:1686-7.  Back to cited text no. 7  [PUBMED]  
8.Guerra R, Cavallini GM, Longanesia L, Casolari C, Bertoli G, Rivasi F, et al. Rhodotorula glutinis keratitis. Int ophthalmol 1992;16:187-90.  Back to cited text no. 8    
9.Zaas AK, Boyce M, Schell W, Lodge BA, Miller JL, Perfect JR. Risk of fungemia due to Rhodotorula and antifungal susceptibility testing of Rhodotorula isolates. J Clin Microbiol 2003;41:5233-5.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]
10.Tuon FF, de Almeida GM, Costa SF. Central venous catheter-associated fungemia due to Rhodotorula spp. a systematic review. Med Mycol 2007;45:441-7.  Back to cited text no. 10    
11.Diekema DJ, Petroelje B, Messer SA, Hollis RJ, Pfaller MA. Activities of available and investigational antifungal agents against Rhodotorula species. J Clin Microbiol 2005;43:476-8.  Back to cited text no. 11  [PUBMED]  [FULLTEXT]
12.Colle JG, Fraser AG, Marmion BP, Simmon A, editors. Mackie and McCartney Practical Medical Microbiology, 14 th ed. Edinburgh: Churchill Livingstone; 1996; p. 695-717.  Back to cited text no. 12    
13.Hazen KC. New and emerging yeast pathogens. Clin Microbiol Rev 1995;8:462-78.  Back to cited text no. 13  [PUBMED]  [FULLTEXT]
14.Pore RS, Chen J. Meningitis caused by Rhodotorula. Sabouraudia 1976;14:331-5.  Back to cited text no. 14  [PUBMED]  
15.Gomez-Lopez A, Mellado E, Rodriguez-Tudela JL, Cuenca-Estrella M. Susceptibility profile of 29 clinical isolates of Rhodotorula spp. and literature review. J Antimicrob Chemother 2005;55:312-6.  Back to cited text no. 15    


    Figures

  [Figure 1], [Figure 2]


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