Annals of Indian Academy of Neurology
ORIGINAL ARTICLE
Year
: 2018  |  Volume : 21  |  Issue : 4  |  Page : 250--255

Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis


BR Vijaykumar1, R Sai Kant1, C Rajendran2, Swathi U Lekshmi1, Sundar Keerthana1, Anita Mahadevan3, SK Shankar3, RS Jayshree1 
1 Department of Microbiology, Kidwai Cancer Institute, Bengaluru, Karnataka, India
2 Defence Food Research Laboratory, Defence Research and Development Organisation, Siddhartha Nagar, Mysore, Karnataka, India
3 Department of Pathology, National Institute of Mental Health and Neuro Sciences, Bengaluru, Karnataka, India

Correspondence Address:
Prof. R S Jayshree
Department of Microbiology, Kidwai Cancer Institute, Hosur Road, Bengaluru - 560 029, Karnataka
India

Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. Settings and Design: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. Subjects and Methods: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. Results: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes–likely recombinants of the canonical types; one case closely corresponded to Type II genotype. Conclusions: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking.


How to cite this article:
Vijaykumar B R, Kant R S, Rajendran C, Lekshmi SU, Keerthana S, Mahadevan A, Shankar S K, Jayshree R S. Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis.Ann Indian Acad Neurol 2018;21:250-255


How to cite this URL:
Vijaykumar B R, Kant R S, Rajendran C, Lekshmi SU, Keerthana S, Mahadevan A, Shankar S K, Jayshree R S. Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis. Ann Indian Acad Neurol [serial online] 2018 [cited 2020 Jul 7 ];21:250-255
Available from: http://www.annalsofian.org/article.asp?issn=0972-2327;year=2018;volume=21;issue=4;spage=250;epage=255;aulast=Vijaykumar;type=0