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Figure 1: 2D-DIGE based separation of proteins and identifi cation by mass spectrometry. Proteins from two samples are labeled using Cy3 or Cy5 prior to pooling. First dimension separation of proteins is carried out using immobilized pI gradient strip and second dimension separation is carried out on SDS-PAGE gel. Differentially expressed proteins are identifi ed using a fl uorescence scanner and unique spots are subjected to in-gel trypsin digestion, released peptides could be analyzed on LC-MS/MS for further identifi cation

Figure 1: 2D-DIGE based separation of proteins and identifi cation by mass spectrometry. Proteins from two samples are labeled using Cy3 or Cy5 prior to pooling. First dimension separation of proteins is carried out using immobilized pI gradient strip and second dimension separation is carried out on SDS-PAGE gel. Differentially expressed proteins are identifi ed using a fl uorescence scanner and unique spots are subjected to in-gel trypsin digestion, released peptides could be analyzed on LC-MS/MS for further identifi cation