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Figure 2: Western blot showing dysferlin protein expression. Immunoblotting analysis of dysferlin protein expression. Protein lysate from control as well as patient monocytes analyzed for dysferlin expression using NCL-hamlet antibody specific for 235 kDa dysferlin protein. Lane 2 and 5 control lysate shows an intense band. Lane 4, 6 and 8 shows absence of dysferlin protein in TDM-146 (JF tool results 97.34% probability with high concordance), TDM-147 (JF tool results 97.28% probability with high concordance), TDM-149 (JF tool results 64.9% probability with medium concordance) respectively. Lane 3 and 7 shows presence of dysferlin protein in TDM-145 (JF tool result 52.2% probability with low concordance) and TDM-148 (JF tool results 95.11% probability with high concordance). GAPDH used as a loading control (37 kDa)

Figure 2: Western blot showing <i>dysferlin</i> protein expression. Immunoblotting analysis of <i>dysferlin</i> protein expression. Protein lysate from control as well as patient monocytes analyzed for <i>dysferlin</i> expression using NCL-hamlet antibody specific for 235 kDa <i>dysferlin</i> protein. Lane 2 and 5 control lysate shows an intense band. Lane 4, 6 and 8 shows absence of <i>dysferlin</i> protein in TDM-146 (JF tool results 97.34% probability with high concordance), TDM-147 (JF tool results 97.28% probability with high concordance), TDM-149 (JF tool results 64.9% probability with medium concordance) respectively. Lane 3 and 7 shows presence of <i>dysferlin</i> protein in TDM-145 (JF tool result 52.2% probability with low concordance) and TDM-148 (JF tool results 95.11% probability with high concordance). GAPDH used as a loading control (37 kDa)